A thermodynamic analysis of mitotic spindle equilibrium at active metaphase.

@article{Stephens1973ATA,
  title={A thermodynamic analysis of mitotic spindle equilibrium at active metaphase.},
  author={Raymond E Stephens},
  journal={The Journal of cell biology},
  year={1973},
  volume={57 1},
  pages={133-47}
}
The mitotic apparatus of first-division metaphase eggs of the sea urchin Strongylocentrotus drobachiensis was observed by means of polarization microscopy under controlled temperature conditions. Eggs were fertilized and grown at two temperature extremes in order to produce two different sizes of available spindle pool. Slow division time allowed successive samples of such cells to be observed at the same point in metaphase but at different equilibrium temperatures, yielding curves of metaphase… CONTINUE READING
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