A tetracycline-inducible gene expression system in Entamoeba histolytica.

  title={A tetracycline-inducible gene expression system in Entamoeba histolytica.},
  author={Girija Ramakrishnan and R R Vines and Barbara J. Mann and William A Petri},
  journal={Molecular and biochemical parasitology},
  volume={84 1},
We have developed an episomal inducible gene expression system in Entamoeba histolytica based on the TetR repressor. The tetR gene was placed under control of 5' and 3' ferredoxin (fdx) regulatory sequences on a plasmid encoding the hygromycin resistance gene directed by 5' and 3' hgl sequences. The reporter luciferase constructs were introduced on a second episome bearing the neomycin resistance gene controlled by 5' and 3' actin sequences. The reporter constructs were driven by the hgl5… CONTINUE READING

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