A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range.

@article{Santoro1992ATO,
  title={A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range.},
  author={Marcelo Matos Santoro and David Wayne Bolen},
  journal={Biochemistry},
  year={1992},
  volume={31 20},
  pages={4901-7}
}
Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conventional analysis of GdnHCl unfolding… CONTINUE READING