A terminal antibody method based on multiple factors that influence ELISA results for measurement of antibody affinity in clinical specimens.

OBJECTIVE To establish a new method for the measurement of antibody affinity in clinical samples. METHODS Serial dilutions of antiserum samples were prepared to find the threshold concentration of antibody separating detectable from negative ELISA results. This threshold concentration was defined as the terminal antibody (TA) concentration, and a new… (More)