A temperature and salt-tolerant L-glutaminase from gangotri region of uttarakhand himalaya: enzyme purification and characterization.


Purification and characterization of halotolerant, thermostable alkaline L-glutaminase from a Bacillus sp. LKG-01 (MTCC 10401), isolated from Gangotri region of Uttarakhand Himalaya, is being reported in this paper. Enzyme has been purified 49-fold from cell-free extract with 25% recovery (specific activity 584.2 U/mg protein) by (NH₄)₂SO₄ precipitation… (More)
DOI: 10.1007/s12010-012-9576-0


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