A technique for the simultaneous staining of both nucleolar organizer regions and kinetochores of human chromosomes with silver.

Abstract

Pretreatment of human metaphase chromosomes with NaOH at a pH of 8.5, followed by staining with silver nitrate, differentially stains both the nucleolar organizer regions on the 10 acrocentric chromosomes as well as the kinetochore centers on all 46 chromosomes. 

Topics

Cite this paper

@article{Denton1977ATF, title={A technique for the simultaneous staining of both nucleolar organizer regions and kinetochores of human chromosomes with silver.}, author={Thomas E. Denton and W R Brooke and William Mike Howell}, journal={Stain technology}, year={1977}, volume={52 6}, pages={311-3} }