• Corpus ID: 35400260

A technique for the histochemical demonstration of polyphenol oxidase and its application to egg-shell formation in helminths and byssus formation in Mytilus

@article{Smyth1954ATF,
  title={A technique for the histochemical demonstration of polyphenol oxidase and its application to egg-shell formation in helminths and byssus formation in Mytilus},
  author={James D. Smyth},
  journal={Journal of Cell Science},
  year={1954},
  volume={95},
  pages={139-152}
}
  • J. D. Smyth
  • Published 1 June 1954
  • Biology
  • Journal of Cell Science
1. The distribution of polyphenol oxidase in quinone-tanning systems may be demonstrated in frozen-dried sections by incubation in 0.2 per cent, aqueous catechol at 400° C. for 15-60 minutes. A red colour develops at the enzyme site. 2. The evidence for the view that the egg-shell in trematodes, in certain cestode groups, and in turbellarians, is a quinone-tanned protein secreted by the so-called ‘vitelline’ glands, is summarized. The ‘vitelline’ cells, in addition to giving positive reactions… 

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