A technique for simultaneous measurement of Ca2+, FRET fluorescence and force in intact mouse small arteries.

@article{Wier2008ATF,
  title={A technique for simultaneous measurement of Ca2+, FRET fluorescence and force in intact mouse small arteries.},
  author={Withrow Gil Wier and Mark A Rizzo and Hema Raina and Joseph Zacharia},
  journal={The Journal of physiology},
  year={2008},
  volume={586 10},
  pages={2437-43}
}
FRET (Forster resonance energy transfer)-based biosensor molecules are powerful tools to reveal specific molecular interactions in cells. Typically however, they are used in cultured cells that (inevitably) express different genes than their counterparts in intact organisms. In such cells it may be impossible to administer physiological stimuli and measure physiological outputs. Here, through the use of transgenic mice that express a FRET-based myosin light chain kinase (MLCK) biosensor… CONTINUE READING