A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry.

@article{Shi1992ATF,
  title={A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry.},
  author={Shan-rong Shi and Claude C{\^o}t{\'e} and Krishan L Kalra and Clive R. Taylor and Atul K. Tandon},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1992},
  volume={40 6},
  pages={787-92}
}
The application of immunohistochemistry to routinely decalcified, celloidin-embedded human temporal bone sections has been hampered because of antigen loss during processing of the specimens. To our knowledge, there has been no published report to date describing immunohistochemical staining of such tissues suitable for examination by light microscopy. Here we report a novel antigen retrieval technique which can be successfully used to stain a variety of antigens in routinely formalin-fixed… CONTINUE READING

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