A technique for localizing LDL by immunofluorescence in formalin-fixed and paraffin-embedded atherosclerotic lesions.


A protocol has been developed by which apo beta, the protein component of plasma low density lipoproteins (LDL), can be localized in four micron-thick sections of formalin-fixed and paraffin-embedded arteries with atherosclerotic involvement from man or non-human primate. An immunofluorescence procedure was employed that utilized affinity column-purified… (More)