A targeted metabolomic protocol for short-chain fatty acids and branched-chain amino acids

@article{Zheng2013ATM,
  title={A targeted metabolomic protocol for short-chain fatty acids and branched-chain amino acids},
  author={Xiaojiao Zheng and Yunping Qiu and Wei Zhong and Sarah Baxter and Mingming Su and Qiong Li and Guoxiang Xie and Brandon M. Ore and Shanlei Qiao and Melanie D. Spencer and Steven H Zeisel and Zhanxiang 3 Zhou and A. Jian-Hua Zhao and Wei Jia},
  journal={Metabolomics},
  year={2013},
  volume={9},
  pages={818-827}
}
Research in obesity and metabolic disorders that involve intestinal microbiota demands reliable methods for the precise measurement of the short-chain fatty acids (SCFAs) and branched-chain amino acids (BCAAs) concentration. Here, we report a rapid method of simultaneously determining SCFAs and BCAAs in biological samples using propyl chloroformate (PCF) derivatization followed by gas chromatography–mass spectrometry (GC–MS) analysis. A one-step derivatization using 100 μL of PCF in a reaction… CONTINUE READING
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