A talin fragment as an actin trap visualizing actin flow in chemotaxis, endocytosis, and cytokinesis.

@article{Weber2002ATF,
  title={A talin fragment as an actin trap visualizing actin flow in chemotaxis, endocytosis, and cytokinesis.},
  author={Igor Weber and Jens Niewoehner and Aiping Du and Ursula R{\"o}hrig and G{\"u}nther Gerisch},
  journal={Cell motility and the cytoskeleton},
  year={2002},
  volume={53 2},
  pages={136-49}
}
A C-terminal 63-kDa fragment of talin A from Dictyostelium discoideum forms a slowly dissociating complex with F-actin in vitro. This talin fragment (TalC63) has been tagged with GFP and used as a trap for actin filaments in chemotactic cell movement, endocytosis, and mitotic cell division. TalC63 efficiently sequesters actin filaments in vivo. Its translocation reflects the direction and efficiency of an actin flow. Along the body of a migrating Dictyostelium cell, this flow is directed from… CONTINUE READING
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