A tailed PCR procedure for cost-effective, two-order multiplex sequencing of candidate genes in polyploid plants.

@article{Gholami2012ATP,
  title={A tailed PCR procedure for cost-effective, two-order multiplex sequencing of candidate genes in polyploid plants.},
  author={Mahmood Gholami and Wubishet A Bekele and Joerg Schondelmaier and Rod J Snowdon},
  journal={Plant biotechnology journal},
  year={2012},
  volume={10 6},
  pages={
          635-45
        }
}
Complex polyploid crop genomes can be recalcitrant towards conventional DNA sequencing approaches for allele mining in candidate genes for valuable traits. In the past, this has greatly complicated the transfer of knowledge on promising candidate genes from model plants to even closely related polyploid crops. Next-generation sequencing offers diverse solutions to overcome such difficulties. Here, we present a method for multiplexed 454 sequencing in gene-specific PCR amplicons that can… CONTINUE READING
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Haplotyping and copy

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