A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes


A large-scale mutagenesis screen was performed in Medaka to identify genes acting in diverse developmental processes. Mutations were identified in homozygous F3 progeny derived from ENU-treated founder males. In addition to the morphological inspection of live embryos, other approaches were used to detect abnormalities in organogenesis and in specific cellular processes, including germ cell migration, nerve tract formation, sensory organ differentiation and DNA repair. Among 2031 embryonic lethal mutations identified, 312 causing defects in organogenesis were selected for further analyses. From these, 126 mutations were characterized genetically and assigned to 105 genes. The similarity of the development of Medaka and zebrafish facilitated the comparison of mutant phenotypes, which indicated that many mutations in Medaka cause unique phenotypes so far unrecorded in zebrafish. Even when mutations of the two fish species cause a similar phenotype such as one-eyed-pinhead or parachute, more genes were found in Medaka than in zebrafish that produced the same phenotype when mutated. These observations suggest that many Medaka mutants represent new genes and, therefore, are important complements to the collection of zebrafish mutants that have proven so valuable for exploring genomic function in development.

DOI: 10.1016/j.mod.2004.04.016

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@article{FurutaniSeiki2004ASG, title={A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes}, author={Makoto Furutani-Seiki and Takao Sasado and Chikako Morinaga and Hiroshi Suwa and Katsutoshi Niwa and Hiroki Yoda and Tomonori Deguchi and Yukihiro Hirose and Akihito Yasuoka and Thorsten Henrich and Tomomi Watanabe and Norimasa Iwanami and Daiju Kitagawa and Kota Saito and Satoshi Asaka and Masakazu Osakada and Sanae Kunimatsu and Akihiro Momoi and Harun Elmasri and Christoph Winkler and Mirana Ramialison and Felix Loosli and Rebecca Quiring and Matthias Carl and Clemens Grabher and Sylke Winkler and Filippo Del Bene and Ai Shinomiya and Yasuko Kota and Toshiyuki Yamanaka and Yasuko Okamoto and Katsuhito Takahashi and Takeshi Todo and Keiko Abe and Yousuke Takahama and Minoru Tanaka and Hiroshi Mitani and Toshiaki Katada and Hiroshi Nishina and Noboru Nakajima and Joachim Wittbrodt and Hisato Kondoh}, journal={Mechanisms of Development}, year={2004}, volume={121}, pages={647-658} }