A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system

@inproceedings{Noyes2008ASC,
  title={A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system},
  author={Marcus B. Noyes and Xiangdong Meng and Atsuya Wakabayashi and Saurabh Sinha and Michael H. Brodsky and Scot A. Wolfe},
  booktitle={Nucleic acids research},
  year={2008}
}
Specificity data for groups of transcription factors (TFs) in a common regulatory network can be used to computationally identify the location of cis-regulatory modules in a genome. The primary limitation for this type of analysis is the paucity of specificity data that is available for the majority of TFs. We describe an omega-based bacterial one-hybrid system that provides a rapid method for characterizing DNA-binding specificities on a genome-wide scale. Using this system, 35 members of the… CONTINUE READING
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