A systematic analysis of intronic sequences downstream of 5' splice sites reveals a widespread role for U-rich motifs and TIA1/TIAL1 proteins in alternative splicing regulation.

@article{Aznarez2008ASA,
  title={A systematic analysis of intronic sequences downstream of 5' splice sites reveals a widespread role for U-rich motifs and TIA1/TIAL1 proteins in alternative splicing regulation.},
  author={Isabel Aznarez and Yoseph Barash and Ofer Shai and David He and Julian Zielenski and Lap-chee Tsui and John Parkinson and Brendan J. Frey and Johanna M Rommens and Benjamin J. Blencowe},
  journal={Genome research},
  year={2008},
  volume={18 8},
  pages={1247-58}
}
To identify human intronic sequences associated with 5' splice site recognition, we performed a systematic search for motifs enriched in introns downstream of both constitutive and alternative cassette exons. Significant enrichment was observed for U-rich motifs within 100 nucleotides downstream of 5' splice sites of both classes of exons, with the highest enrichment between positions +6 and +30. Exons adjacent to U-rich intronic motifs contain lower frequencies of exonic splicing enhancers and… CONTINUE READING