A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.

@article{Law1995AST,
  title={A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.},
  author={John Law and Girbe Buist and A. J. Haandrikman and Jan Kok and Gerard A. Venema and Kees J Leenhouts},
  journal={Journal of bacteriology},
  year={1995},
  volume={177 24},
  pages={7011-8}
}
A system for generating chromosomal insertions in lactococci is described. It is based on the conditional replication of lactococcal pWV01-derived Ori+ RepA- vector pORI19, containing lacZ alpha and the multiple cloning site of pUC19. Chromosomal AluI fragments of Lactococcus lactis were cloned in pORI19 in RepA+ helper strain Escherichia coli EC101. The frequency of Campbell-type recombinants, following introduction of this plasmid bank into L. lactis (RepA-), was increased by combining the… CONTINUE READING
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