A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins.

@article{Amaratunga1996ASM,
  title={A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins.},
  author={Mohan Amaratunga and Kerry Fluhr and Joseph T. Jarrett and Catherine L Drennan and Martha L. Ludwig and Rowena G. Matthews and Jeffrey D Scholten},
  journal={Biochemistry},
  year={1996},
  volume={35 7},
  pages={2453-63}
}
Cobalamin-dependent methionine synthase from Escherichia coli is a monomeric 136 kDa protein composed of multiple functional regions. The X-ray structure of the cobalamin-binding region of methionine synthase reveals that the cofactor is sandwiched between an alpha-helical domain that contacts the upper face of the cobalamin and an alpha/beta (Rossmann) domain that interacts with the lower face. An unexpected conformational change accompanies binding of the methylcobalamin cofactor. The… CONTINUE READING

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In order to facilitate studies of the roles of amino acid residues in the cobalamin - binding region of methionine synthase , we have constructed a synthetic module corresponding to nucleotides ( nt ) 1741 - 2668 in the metH gene and incorporated it into the wild - type metH gene .
In order to facilitate studies of the roles of amino acid residues in the cobalamin - binding region of methionine synthase , we have constructed a synthetic module corresponding to nucleotides ( nt ) 1741 - 2668 in the metH gene and incorporated it into the wild - type metH gene .
In order to facilitate studies of the roles of amino acid residues in the cobalamin - binding region of methionine synthase , we have constructed a synthetic module corresponding to nucleotides ( nt ) 1741 - 2668 in the metH gene and incorporated it into the wild - type metH gene .
Four mutations ( His759Gly , Asp757Glu , Asp757Asn , and Ser810Ala ) alter residues in the hydrogen - bonded network His - Asp - Ser that connects the histidine ligand of the cobalt to solvent .
Four mutations ( His759Gly , Asp757Glu , Asp757Asn , and Ser810Ala ) alter residues in the hydrogen - bonded network His - Asp - Ser that connects the histidine ligand of the cobalt to solvent .
In order to facilitate studies of the roles of amino acid residues in the cobalamin - binding region of methionine synthase , we have constructed a synthetic module corresponding to nucleotides ( nt ) 1741 - 2668 in the metH gene and incorporated it into the wild - type metH gene .
In order to facilitate studies of the roles of amino acid residues in the cobalamin - binding region of methionine synthase , we have constructed a synthetic module corresponding to nucleotides ( nt ) 1741 - 2668 in the metH gene and incorporated it into the wild - type metH gene .
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