A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript.

@article{Dower2004ASA,
  title={A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript.},
  author={Ken Dower and Nicolas Kuperwasser and Houra Merrikh and Michael Rosbash},
  journal={RNA},
  year={2004},
  volume={10 12},
  pages={1888-99}
}
To investigate the role of 3' end formation in yeast mRNA export, we replaced the mRNA cleavage and polyadenylation signal with a self-cleaving hammerhead ribozyme element. The resulting RNA is unadenylated and accumulates near its site of synthesis. Nonetheless, a significant fraction of this RNA reaches the cytoplasm. Nuclear accumulation was relieved by insertion of a stretch of DNA-encoded adenosine residues immediately upstream of the ribozyme element (a synthetic A tail). This indicates… CONTINUE READING
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