A survey to assess the ultrastructural preservation of fixed biological samples after air-drying from tetramethylsilane.

Abstract

A scanning electron microscope (SEM) survey assessed the quality of ultrastructural preservation of a wide range of tissues from the laboratory rat and from a limited number of botanical and fly-larval samples, air-dried from tetramethylsilane (TMS) after chemical fixation and dehydration. Comparisons were made with critical point drying (CPD) and air-drying from acetone. CPD produced good results in all cases. TMS gave acceptable results with 75%, and acetone with 31%, of rat tissues. Air-drying was unacceptable for the botanical and fly-larval samples. It is concluded that air-drying from TMS is generally useful for mammalian tissues in preparation for the SEM.

Cite this paper

@article{Reville1994AST, title={A survey to assess the ultrastructural preservation of fixed biological samples after air-drying from tetramethylsilane.}, author={W J Reville and M M Heapes and Victor R O'Sullivan}, journal={Journal of electron microscopy}, year={1994}, volume={43 2}, pages={111-5} }