A survey of Nicotiana germplasm for resistance to Tomato spotted wilt virus (TSWV)

  title={A survey of Nicotiana germplasm for resistance to Tomato spotted wilt virus (TSWV)},
  author={Dorota Laskowska and Teresa Doroszewska and Anna Depta and Karolina Kursa and Hanna Olszak-Przybyś and Anna Czubacka},
The reaction to Tomato spotted wilt virus (TSWV) was evaluated in 94 accessions of Nicotiana, originating from the Institute of Soil Science and Plant Cultivation tobacco germplasm collection in Puławy, Poland. Tests for resistance were conducted under greenhouse conditions using single TSWV isolate collected from tobacco plantation in Lublin district, Poland. The presence of the virus was verified using DAS-ELISA. SCAR markers associated with TSWV resistance gene were applied. The members of… 
Resistance Response of the Recently Discovered Species Nicotiana mutabilis to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV) Compared to Other Sources of Resistance
This species extends the genetic variation in the genus Nicotiana and, because of its tolerance to PVY and partial resistance to TSWV, it may be a potential source of resistance to these viruses.
Non-Structural Protein NSm of Tomato Spotted Wilt Virus Is an Avirulence Factor Recognized by Resistance Genes of Tobacco and Tomato via Different Elicitor Active Sites
The results demonstrated that RTSW-mediated resistance is triggered by NSm in the same way as by Sw-5b, however, via different elicitor active sites, and an Avr gene-based diagnostic approach was established and used to determine the presence and effectiveness of resistance genes in tobacco.
Exploring and optimising the conditions for virus-induced gene silencing in an ornamental tobacco, Nicotiana alata
This is the first report of gene silencing using VIGS in ornamental tobacco and can be used as an advantageous tool for assessing target gene functions and engineering for desired objectives such as aesthetic factors of ornamental crops.
Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata
In order to elucidate the unexpected fertility recovery of the fertile F1 plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F1 subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N.tabacum.
Morphological and chemical characteristics of doubled haploids of flue-cured tobacco combining resistance to Thielaviopsis basicola and TSWV
Biological tests and field performance of DH revealed potential to overcome the negative effect of coupling between the RSTV-al gene and genes responsible for the morphological deformations, which might be explained by a recombination during microsporogenesis in F1, however the influence of ‘Polalta’-derived RTSW- al gene cannot be excluded.
The Use of Nicotiana Species in Tobacco Improvement
This review examines how and to what extent natural germplasm resources have been utilized in hybridization and introgression experiments over the past century, and focuses on individual Nicotiana species involved in interspecific hybrids with cultivated tobacco produced by sexual and asexual methods.
Plasticity in the self-incompatibility system of cultivated Nicotiana alata
Findings indicate that SI in N. alata is plastic, with fruits set by aging flowers, especially flowers at stage III, where flowers at the end of flowering stages was lower and more fruits were set at the top of the pseudo-raceme.
Cytological abnormalities during pollen development in interspecific hybrids of Nicotiana
Understanding of the cytological mechanisms of hybrid sterility is deepened, and the application of TSWV-resistant resources in cultivated Nicotiana species through hybrid fertility restoration and backcross breeding is facilitated.
Tracing the Lineage of Two Traits Associated with the Coat Protein of the Tombusviridae: Silencing Suppression and HR Elicitation in Nicotiana Species
This analysis illustrates how structural changes that likely occurred in the CP effector of progenitors of the current genera led to either silencing suppressor activity, HR elicitation in select Nicotiana species, or neither trait.
TÓM TẮT: Nghiên cứu tạo giống cây trồng kháng virus bằng công nghệ RNAi là phương pháp hiện đại và hiệu quả trong việc phòng chống các bệnh virus hại thực vật. Trong các công bố trước, chúng tôi đã


Two Field Isolates of Tomato Spotted Wilt Tospovirus Overcome the Hypersensitive Response of a Pepper (Capsicum annuum) Hybrid with Resistance Introgressed from C. chinense PI152225.
This is the first report of field tospovirus isolates typed as TSWV overcoming the hypersensitive response of Capsicum chinense and PI159236, an ability previously found only in closely related viruses: TCSV and GRSV.
Inheritance of a gene for resistance to tomato spotted wilt virus (TSWV) from Lycopersicon peruvianum Mill
The inheritance of TSWV resistance was found to be a single dominant gene, which indicates a 98.7% penetrance of the resistance gene in plants infected four months after the inoculations.
Tomato Spotted Wilt Virus (TSWV) resistance in tomato derived from Lycopersicon chilense Dun. LA 1938
Results of the field study clearly establish that TSWVfield resistance is present in the Fla 925-2 (Y118)derived lines, and has the distinct characteristic of often `recovering' from initially high levels of virus titer in the tissue to levels below detection with ELISA.
Hypersensitive resistance to tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains
Serological analysis confirmed that nine viral isolates obtained from individual plants with systemic symptoms were backinoculated to the TSWV species (serogroup I), and the susceptible plants in the allelism tests could not be interpreted as possessing a recombinant genotype because of the virulence change in the viral strain.
Differential Response of Selected Peanut (Arachis hypogaea) Genotypes to Mechanical Inoculation by Tomato spotted wilt virus.
This is the first report of differential response of peanut genotypes to TSWV using mechanical inoculation, and the four genotypes should be useful as reference standards for the initial screening and identification of sources of T SWV resistance in peanut germ plasm.
Tomato spotted wilt virus (TSWV) in Greece: its incidence following the expansion of Frankliniella occidentalis, and characterisation of isolates collected from various hosts
Leaf samples were collected from plants with tospovirus-like symptoms from various hosts in different regions of Greece where Thrips tabaci, Frankliniella occidentalis or both vectors occur, and host range studies showed differences in susceptibility.
Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense
  • L. Boiteux
  • Biology, Medicine
    Theoretical and Applied Genetics
  • 2004
The absence of segregation for resistance to TSWV that was observed in all generations of the crosses between C. chinense lines indicated that either a tightly linked group of genes exists or that the resistance is governed by the same single major gene (probably the already described Tsw gene).
Nucleocapsid Gene-Mediated Transgenic Resistance Provides Protection Against Tomato spotted wilt virus Epidemics in the Field.
It is concluded that transgenic resistance to TSWV is effective in reducing incidence of the disease in the field, and that accumulation of transgene protein may be important in broad-spectrum resistance.
AFLP and SCAR Markers Linked to Tomato Spotted Wilt Virus Resistance in Tobacco
By selecting for resistant plants with an improved plant type and marker profile in a BC 3 population, four plants were identified that were missing 1 to 14 of the AFLP markers associated with the alien chromatin, demonstrating that rare recombination events can be identified.
Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense ‘PI 159236’
Segregation ratios strongly indicated that the resistant response to TSWV fits a single-dominant gene model, and the resistance governed by the Tsw gene was not effective against isolates belonging to tomato chlorotic spot virus and groundnut ring spot virus, two previously described Tospovirus species.