A surrogate method for assessment of beta(2)-integrin-dependent adhesion of human eosinophils to ICAM-1.

@article{Zhu2000ASM,
  title={A surrogate method for assessment of beta(2)-integrin-dependent adhesion of human eosinophils to ICAM-1.},
  author={Xiaolei Zhu and Ram Subbaraman and Hiroyuki Sano and Benedikt Jacobs and Akiko Sano and Evan E. Boetticher and Nilda M. Mu{\~n}oz and Alan R. Leff},
  journal={Journal of immunological methods},
  year={2000},
  volume={240 1-2},
  pages={157-64}
}
We have developed and validated an inexpensive and equivalent method for measuring eosinophil adhesion by beta(2)-integrin to endothelial ICAM-1 using bovine serum albumin (BSA) as a surrogate for the immunoglobulin supergene. The number of adherent eosinophils on BSA or ICAM-1 coated microplates was quantified by residual eosinophil peroxidase activity. Non-stimulated eosinophils did not adhere to either BSA or ICAM-1. However, after IL-5 stimulation, either BSA or ICAM-1 caused comparable and… CONTINUE READING
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