A surprising effect of extraction conditions on the mobility of some plant virus coat proteins in SDS-PAGE.

Abstract

The coat protein from purified particles of pea seedborne mosaic potyvirus (PSbMV) moves in SDS-PAGE with an apparent molecular weight (M(r)) of 36 kDa. However, extracts of PSbMV infected plants prepared with SDS or urea contain PSbMV immunoreactive proteins with apparent M(r) 39 kDa as well as 36 kDa. The low mobility form may be generated from the… (More)

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@article{Hassan1994ASE, title={A surprising effect of extraction conditions on the mobility of some plant virus coat proteins in SDS-PAGE.}, author={Hamina Hassan and Bernhard Borkhardt and M. Albrechtsen}, journal={Journal of virological methods}, year={1994}, volume={46 2}, pages={255-61} }