A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A.

@article{Wear2005ASP,
  title={A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A.},
  author={Martin A Wear and Alan Patterson and Kirk J. Malone and Colin J Dunsmore and Nicholas J. Turner and Malcolm D. Walkinshaw},
  journal={Analytical biochemistry},
  year={2005},
  volume={345 2},
  pages={214-26}
}
A simple protocol for generating a highly stable and active surface plasmon resonance (SPR) sensor surface of recombinant human hexahistidine cyclophilin A (His-CypA) is described. The sensor surface was sensitive and stable enough to allow, for the first time, the screening and ranking of several novel small-molecule (Mr approximately 250-500 Da) ligands in a competition binding assay with cyclosporin A (CsA). It also allowed us to accurately determine the kinetic rate constants for the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Spongionella Secondary Metabolites Regulate Store Operated Calcium Entry Modulating Mitochondrial Functioning in SH-SY5Y Neuroblastoma Cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology • 2015

Similar Papers

Loading similar papers…