A superfusion system to study border zones in confluent cultures of neonatal rat heart cells.

@article{Hyatt1998ASS,
  title={A superfusion system to study border zones in confluent cultures of neonatal rat heart cells.},
  author={C J Hyatt and John J. Lemasters and Barbara J. Muller-Borer and Timothy A. Johnson and Wayne E. Cascio},
  journal={The American journal of physiology},
  year={1998},
  volume={274 6},
  pages={
          H2001-8
        }
}
We present a new experimental method to study intracellular ion regulation in cultured cardiomyocytes at a border zone separating two different and distinct environments. Our system uses a dual-flow superfusion chamber to produce two different but adjacent environments over a monolayer of cardiomyocytes. Fluorescent microscopy of fluorescein showed that the transition between the two environments was nearly linear and was 220-320 micron wide depending on fluid viscosity and velocity. We… CONTINUE READING

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