A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.

@article{Biedendieck2007ASP,
  title={A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.},
  author={Rebekka Biedendieck and Martin Gamer and Lothar J{\"a}nsch and Steffen Meyer and Manfred Rohde and W D Deckwer and Dieter Jahn},
  journal={Journal of biotechnology},
  year={2007},
  volume={132 4},
  pages={426-30}
}
A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P(sacB) allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose… CONTINUE READING

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