A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases.

@article{Minic2007ASO,
  title={A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases.},
  author={Zoran Minic and Elisabeth Jamet and Luc N{\'e}groni and P Arsene der Garabedian and Michel Zivy and Lise Jouanin},
  journal={Journal of experimental botany},
  year={2007},
  volume={58 10},
  pages={2503-12}
}
N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using nanoHPLC-MS/MS and MALDI-TOF MS. 102 glycoproteins were identified. 94% of these proteins were predicted by bioinformatics to be targeted to the secretory pathway and 87% of them were predicted to be localized in the cell wall or at the plasma membrane. 30% of these proteins belong to glycoside hydrolase (GH… CONTINUE READING