A study of the sensitivity and specificity of four presumptive tests for blood.

@article{Cox1991ASO,
  title={A study of the sensitivity and specificity of four presumptive tests for blood.},
  author={Matthew Cox},
  journal={Journal of forensic sciences},
  year={1991},
  volume={36 5},
  pages={
          1503-11
        }
}
  • M. Cox
  • Published 1 September 1991
  • Medicine
  • Journal of forensic sciences
The purpose of this work was to conduct a comparative study of the sensitivity and specificity of phenolphthalein, tetramethylbenzidine, leucomalachite green, and orthotolidine as presumptive tests for blood. The findings of this study indicate that the phenolphthalein and the leucomalachite green tests are the most specific and that the tetramethylbenzidine and orthotolidine tests are the most sensitive of the group. The author concludes that the phenolphthalein test is the best single test… 
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Overall, luminol was the most effective method, also providing information on bloodstain patterns, and was the preferred test for diluted blood having the highest sensitivity, NPV, and the lowest OMR.
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Luminol should be considered as a “preferable” or “first choice” reagent for use in presumptive tests due to a higher improbability of producing false positives and false negatives, apart from being safer than other substances.
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The aim of this study was to validate the two presumptive blood tests LMG, LCV and the three visualising blood methods Bluestar Forensics, Lumiscene and the Ruhoff method. The methods’ sensitivity,
Comparative study of presumptive and confirmatory tests for detection of blood on serial dilutions and washed stains
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Luminol is the most sensitive presumptive test for detecting blood on washed and diluted bloodstains, and sensitivity decreases with an increase in wash cycles.
Validation of presumptive tests for non-human blood using Kastle Meyer and Hemastix reagents.
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Owing to its ease of use, higher sensitivity, and lack of interference with downstream DNA analysis, and despite its reduced specificity compared to Kastle Meyer, the Hemastix method is more appropriate for use in wildlife crime investigations.
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References

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A STUDY OF THE SENSITIVITY, STABILITY AND SPECIFICITY OF PHENOLPHTHALEIN AS AN INDICATOR TEST FOR BLOOD
TLDR
Results indicate that the three-stage phenolphthalein presumptive test for blood appears to be somewhat less sensitive than the conventional benzidine test, but results indicate that it has several advantages in terms of specificity and stability.
An evaluation of tetramethylbenzidine as a presumptive test for blood.
TLDR
In forensic use, a presumptive test indicating the possible presence of blood is an invaluable tool in screening out samples that are definitely not blood and do not require further testing.
Effect of fabric washing on the presumptive identification of bloodstains.
  • M. Cox
  • Chemistry, Medicine
    Journal of forensic sciences
  • 1990
TLDR
The findings of this study indicate that the retention of bloodstains on washed fabrics depends upon the particular fiber composition of the fabric, the specific blood screening test used, and whether or not a detergent was used in the wash.
The identification of human blood stains: a critical survey