A study of the protein secretory pathway of Aspergillus niger using a glucoamylase-GFP fusion protein.

@article{Khalaj2001ASO,
  title={A study of the protein secretory pathway of Aspergillus niger using a glucoamylase-GFP fusion protein.},
  author={V Khalaj and Jayne L Brookman and G. D. Robson},
  journal={Fungal genetics and biology : FG & B},
  year={2001},
  volume={32 1},
  pages={55-65}
}
The effect of various treatments that block protein secretion was visualized in Aspergillus niger using a strain expressing a glucoamylase-GFP fusion protein. Cold shock caused the retention of the fusion protein in a reticulate network (ER) with brighter nodes that may represent Golgi bodies. Treatment of germlings with brefeldin A (BFA) also initially caused accumulation within the ER but prolonged exposure led to the formation and targeting of the fusion protein to vacuoles from the ER… CONTINUE READING

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