A study of polynucleotide phosphorylase production by Escherichia coli in a hollow fibre reactor

@article{Nie2005ASO,
  title={A study of polynucleotide phosphorylase production by Escherichia coli in a hollow fibre reactor},
  author={S. Nie and Lin Ma and L. Du and B. Han},
  journal={World Journal of Microbiology and Biotechnology},
  year={2005},
  volume={21},
  pages={424-428}
}
  • S. Nie, Lin Ma, +1 author B. Han
  • Published 2005
  • Biology
  • World Journal of Microbiology and Biotechnology
A 30-l hollow fibre reactor with continuous fermentation for cell recycling of Escherichia coli AS 1.183 was used to remove the inhibitory effects on cell growth and extend the fast growth phase to increase the yield of polynucleotide phosphorylase (PNPase) in E. coli cells. When the dilution rate was 1.5 h−1, the cell concentration of E. coli reached 235 g/l (wet wt, 70% moisture content), with PNPase activity above 90 u/g (wet wt). With the dilution rate is 1.0 h−1, the fermentor volumetric… Expand

References

SHOWING 1-10 OF 13 REFERENCES
Continuous production of lactic acid in a cell recycle reactor
TLDR
The production of lactic acid from glucose has been demonstrated using a CSTR (continuous stirred-tank reactor) with cell recycle using lactobacillus delbrueckii and at steady state the net cell growth appeared to be negligible. Expand
Overexpression and purification of untagged polynucleotide phosphorylases.
TLDR
The development of new, straightforward procedures for the purification of bacterial polynucleotide phosphorylases (PNPases) are reported, and it is suggested that the approaches described here may be applied generally to the overexpression and purifying of eubacterial polyn nucleotide phosphorolysis. Expand
A model for micro/ultrafiltration cell deactivation in cell-recycle reactors
Mechanical stress due to micro/ultrafiltration has been suggested by a number of authors as one of the sources for cell deactivation in continuous cell-recycle reactors. This work introduces cellExpand
High‐concentration cultivation of Lactococcus cremoris in a cell‐recycle reactor
TLDR
High‐cell concentration cultivation of Lactococcus cremoris, a homofermentative lactic acid producer, in a cell‐recycle fermentor and the dependence of growth characteristics on operating conditions was identified and quantified using lactose as the carbon source. Expand
High density cell culture by membrane-based cell recycle.
TLDR
A theoretical of production of primary and secondary metabolites in membrane-based recycle systems is presented and Operation of this type of system is discussed with examples from aerobic and anaerobic fermentations. Expand
Growth ofschizosaccharomyces pombe on glucose-malte mixtures in continuous cell-recycle cultures kinetics of substrate utilization
TLDR
The aerobic growth ofSchizosaccharomyces pombe on mixtures of glucose and malate was investigated during continuous high cell density cultures with partial cell-recycle using a membrane bioreactor and the mass balance analysis revealed the incorporation of malic acid into biomass. Expand
Optimization of process design for continuous ethanol production by Zymomonas mobilis ATCC 29191
A two-stage continuous-stirred-tank-reactor (CSTR) system is used to achieve high final alcohol concentrations (>100 g/l) with Zymononas mobilis ATCC 29191. By employing continuous cell recycle onExpand
Ethanol production in a microporous hollow‐fiber‐based extractive fermentor with immobilized yeast
TLDR
The significant productivity increase with in situ solvent extraction became more evident as solvent/ substrate flow ratio increased, and a model of the locally integrated extractive bioreactor describes the observed fermentor performance quite well. Expand
Determination of maintenance coefficients of Saccharomyces cerevisiae cultures with cell recycle by cross‐flow membrane filtration
The fermentation of glucose by a strain of Saccharomyces cerevisiae was studied in a continuous single‐stage process with recycle of the cells via cross‐flow micro‐filtration membranes. OperatingExpand
17 Polynucleotide Phosphorylase
Publisher Summary Polynucleotide phosphorylase (PNPase) is the first enzyme that can catalyze the formation of polyribonucleotides with a 3′,5′-phosphodiester bond. In the forward reaction, longExpand
...
1
2
...