A study of polynucleotide phosphorylase production by Escherichia coli in a hollow fibre reactor

  title={A study of polynucleotide phosphorylase production by Escherichia coli in a hollow fibre reactor},
  author={S. Nie and Lin Ma and L. Du and B. Han},
  journal={World Journal of Microbiology and Biotechnology},
  • S. Nie, Lin Ma, +1 author B. Han
  • Published 2005
  • Biology
  • World Journal of Microbiology and Biotechnology
A 30-l hollow fibre reactor with continuous fermentation for cell recycling of Escherichia coli AS 1.183 was used to remove the inhibitory effects on cell growth and extend the fast growth phase to increase the yield of polynucleotide phosphorylase (PNPase) in E. coli cells. When the dilution rate was 1.5 h−1, the cell concentration of E. coli reached 235 g/l (wet wt, 70% moisture content), with PNPase activity above 90 u/g (wet wt). With the dilution rate is 1.0 h−1, the fermentor volumetric… Expand


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