A study of non-isotopic in situ hybridization histochemistry on postmortem changes in vasopressin mRNA in rat brain

@article{Arai1989ASO,
  title={A study of non-isotopic in situ hybridization histochemistry on postmortem changes in vasopressin mRNA in rat brain},
  author={H. i 24 Arrizabalaga Arai and Iwahide Noguchi and Naoki Sagi and Takashi Moroji and Reiji Iizuka},
  journal={Neuroscience Letters},
  year={1989},
  volume={103},
  pages={127-132}
}
Rat models which stimulated various postmortem conditions were used to determine postmortem changes in the vasopressin mRNA content of the brain. In situ hybridization histochemistry experiments using biotinylated oligonucleotide probes revealed that vasopressin mRNA could be detected in the rat hypothalamic nuclei, even though the rats had been killed and left for 8 h postmortem at room temperature and the brains were then fixed by immersion in 4% paraformaldehyde instead of by transcardial… CONTINUE READING

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