A study of insulin receptors in human mononuclear leucocytes.

  title={A study of insulin receptors in human mononuclear leucocytes.},
  author={Oluf Pedersen and Henning Beck-Nielsen},
  journal={Acta endocrinologica},
  volume={83 3},
Insulin binding sites were demonstrated in human mononuclear leucocytes by use of a technique which includes isolation of mononuclear leucocytes from defibrinated blood and separation of cell bound and free [125I]insulin with silicone oil. The binding was time and temperature dependent. At 15 degrees C equilibrium was reached after 90 min and a plateau maintained for at least 50 min. Incubations were carried out at 4 degrees C, 15 degrees C and 37 degrees C. Maximal binding was obtained at 15… CONTINUE READING