A study of genetic variation at Est-alpha, Est-beta, A my, Acph and alpha-Gpdh loci in local samples of Hefei, Wuhu, Jiujiang, Nanchang, Changzhou, Fuzhou and Quanzhou Drosophila virilis population is presented. The employment of standard techniques of polyacrylamide electrophoresis and agarose electrophoresis reveals that the former three loci are highly polymorphic in all these populations. According to the allelic frequency of the 5 loci, a genetic distance between the above-mentioned populations was calculated. Along with the results obtained by Mr He Chaozheng from Hongze, Nanjing, Hangzhou and Ningbo populations, a dendrogram was made. It was found that Quanzhou and Fuzhou populations differed from the others in allelic frequencies and genetic distances which suggests that this difference is related to the extent of geographic isolation among them.