A study of copulation, sporulation and meiotic segregation in Candida lipolytica

  title={A study of copulation, sporulation and meiotic segregation in Candida lipolytica},
  author={Claude M. Gaillardin and V{\'e}ronique Charoy and Henri Heslot},
  journal={Archiv f{\"u}r Mikrobiologie},
We have attempted to optimize conjugation and sporulation in Candida lipolytica, by studying the conditions of culture and growth. Copulation between compatible strains is a rare event, particularly in the case of auxotrophic mutants. However, diploids can be selected for on minimal medium provided parents are suitable auxotrophs. These diploids can multiply vegetatively for many generations. They can also be induced to sporulate at a very high frequency. Free ascospores were isolated by means… CONTINUE READING
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