A study in microtomy for electron microscopy

  title={A study in microtomy for electron microscopy},
  author={Keith R. Porter and Josef Blum},
  journal={The Anatomical Record},

Transcriptomic and Physiological Insights into the Robustness of Long Filamentous Cells of Methanosaeta harundinacea, Prevalent in Upflow Anaerobic Sludge Blanket Granules

The genetic basis for the prevalence of the long filamentous morphology of M. harundinacea cells in upflow anaerobic sludge blanket granules is revealed, which implies that the short filaments can be stressed.

Morphomics via Next-generation Electron Microscopy

This paper presents a meta-analysis of Mouse Phenotype Analysis for Cancer Genomics and its Applications in Health Science Innovation and Molecular Oncology that shows clear trends in prognosis and in particular in women with high Mayer-Leroy Mayer disease.

Axonal Organelles as Molecular Platforms for Axon Growth and Regeneration after Injury

This review summarizes recent literature highlighting a central role for organelles such as recycling endosomes, the endoplasmic reticulum, mitochondria, lysosome, autophagosomes and the proteasome in developmental axon growth, and describes how they can be targeted to promote axon regeneration after injury to the adult CNS.

Personal reminiscences of early days in electron microscopy

  • D. Drummond
  • Materials Science
    The Beginnings of Electron Microscopy - Part 1
  • 2021

The Locus Coeruleus- Norepinephrine System in Stress and Arousal: Unraveling Historical, Current, and Future Perspectives

This work discusses technological advancements that chronologically led to the current understanding of the arousal system, focusing on the multifaceted nucleus locus coeruleus.

A brief history of how microscopic studies led to the elucidation of the 3D architecture and macromolecular organization of higher plant thylakoids

The most recent discoveries made possible by atomic force microscopy of hydrated membranes, and electron tomography and cryo-electron tomography of cryofixed thylakoids have provided novel insights intoThylakoid architecture and plastoglobules, while also producing molecular-scale views of grana and stroma thylkoids in which individual functional complexes can be identified.

A perfect confluence of physiology and morphology: discovery of the transverse tubular system and inward spread of activation in skeletal muscle.

  • J. Rall
  • Biology
    Advances in physiology education
  • 2020
It was the confluence of physiology and morphology that brought clarity and a major advance in understanding, leading to the discovery of the transverse tubular system and inward spread of activation in skeletal muscle.

SBEM Techniques




A Cantilever Microtome for Precision Sectioning in Electron and Light Microscopy

A microtome has been built incorporating the principle of deflection of a cantilever to provide the highly reproducible motion necessary in cutting thin sections for electron microscopy. The

An Objective for Use in the Electron Microscopy of Ultra‐Thin Sections

An objective has been designed which is particularly suitable for the study of tissue sections. It consists of a large opening polepiece system operated at a focal length of 12 mm. It includes a

On the Sharpening of Microtome Knives for Ultra‐Thin Sectioning

Conventional methods of sharpening involve extensive grinding which is primarily for the removal of large nicks but which serves the additional purpose of fitting the knife to the sharpening means.

Improved Ultra‐Thin Sectioning of Tissue for Electron Microscopy

The use of a reservoir of liquid to collect ribbons of thin sections as they are cut enables serial sections to be obtained and provides means for detecting variations in thickness. Reducing the

Sectioning Techniques for Electron Microscopy Using a Conventional Microtome

  • D. PeaseR. Baker
  • Biology
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1948
Conventional histological techniques have been modified so that it is possible consistently to cut 0.2 micron sections for use with the electron microscope, and micrographs of rat liver sections show that the principal artefacts are due to fixation rather than subsequent treatments.

A new microtome for thin-sectioning for electron microscopy.