A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis.

@article{Boisbouvier1998ASH,
  title={A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis.},
  author={J{\'e}r{\^o}me Boisbouvier and J P Albrand and Martin Blackledge and Michel Jaquinod and H Schweitz and Michael Lazdunski and Dominique Marion},
  journal={Journal of molecular biology},
  year={1998},
  volume={283 1},
  pages={205-19}
}
The solution structure of mamba intestinal toxin 1 (MIT1), isolated from Dendroaspis polylepis polylepis venom, has been determined. This molecule is a cysteine-rich polypeptide exhibiting no recognised family membership. Resistance to MIT1 to classical specific endoproteases produced contradictory NMR and biochemical information concerning disulphide-bridge topology. We have used distance restraints allowing ambiguous partners between S atoms in combination with NMR-derived structural… CONTINUE READING