A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase beta.

@article{Pelletier1996ASB,
  title={A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase beta.},
  author={Hugues Pelletier and Michael R. Sawaya and William T Wolfle and Samuel H. Wilson and Joseph Kraut},
  journal={Biochemistry},
  year={1996},
  volume={35 39},
  pages={12762-77}
}
When crystals of human DNA polymerase beta (pol beta) complexed with DNA [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H., & Kraut, J. (1996) Biochemistry 35, 12742-12761] are soaked in the presence of dATP and Mn2+, X-ray structural analysis shows that nucleotidyl transfer to the primer 3'-OH takes place directly in the crystals, even though the DNA is blunt-ended at the active site. Under similar crystal-soaking conditions, there is no evidence for a reaction when Mn2+ is replaced by… CONTINUE READING

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