A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.

@article{Flors2007ASA,
  title={A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.},
  author={Cristina Flors and Jun-ichi Hotta and Hiroshi Uji-i and Peter Dedecker and Ryoko Ando and Hideaki Mizuno and Atsushi Miyawaki and Johan Hofkens},
  journal={Journal of the American Chemical Society},
  year={2007},
  volume={129 45},
  pages={13970-7}
}
The photophysical properties and photoswitching scheme of the reversible photoswitchable green fluorescent protein-like fluorescent proteins Dronpa-2 and Dronpa-3 were investigated by means of ensemble and single-molecule fluorescence spectroscopy and compared to those of the precursor protein Dronpa. The faster response to light and the faster dark recovery of the new mutants observed in bulk also hold at the single-molecule level. Analysis of the single-molecule traces allows us to extract… CONTINUE READING

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A stroboscopic approach for fast photoactivation - localization microscopy with Dronpa mutants

  • J. Hotta, H. Uji-I, +4 authors J. Hofkens

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