A strategy for increasing an in vivo flux by genetic manipulations. The tryptophan system of yeast.

@article{Niederberger1992ASF,
  title={A strategy for increasing an in vivo flux by genetic manipulations. The tryptophan system of yeast.},
  author={Peter Niederberger and Roopa Prasad and Giuseppe Miozzari and Henrik Kacser},
  journal={The Biochemical journal},
  year={1992},
  volume={287 ( Pt 2)},
  pages={
          473-9
        }
}
Decreases in enzyme activity often have little effect on the flux carried by the pathway. Similarly, up-modulation of single genes, and hence of the dependent enzyme concentrations, is frequently found to be ineffective in increasing the flux in the pathway in which the enzyme occurs. This insensitivity to enzyme variation is demonstrated experimentally for five separate enzymes in the tryptophan synthesis system of yeast, first by down-modulation of the gene dose and secondly by increasing the… CONTINUE READING
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