A strategy for identification and quantification of detergents frequently used in the purification of membrane proteins.

@article{Eriks2003ASF,
  title={A strategy for identification and quantification of detergents frequently used in the purification of membrane proteins.},
  author={Laura R Eriks and June A. Mayor and Ronald S. Kaplan},
  journal={Analytical biochemistry},
  year={2003},
  volume={323 2},
  pages={234-41}
}
A methodology that enables the identification and quantification of detergents frequently used in the purification of membrane proteins has been developed. The procedure consists of detergent separation via thin-layer chromatography, followed by visualization with iodine vapor staining and subsequent quantification with laser densitometry. We demonstrate that a panel of detergents that are frequently used to purify membrane proteins displays distinctive mobilities in a solvent system consisting… CONTINUE READING