A strategy for enrichment of the bioactive sphingoid base-1-phosphates produced by Hypericum perforatum L. in a balloon type airlift reactor.

Abstract

An efficient enrichment method using immobilized metal affinity chromatography (IMAC) was developed for selective extraction of bioactive sphingoid base-1-phosphates (SB1Ps) from adventitious roots of Hypericum perforatum cultured in bioreactor. The phosphate-selective IMAC enrichment coupled with LC-MS/MS enabled sensitive analysis of low-abundance SB1Ps… (More)
DOI: 10.1016/j.biortech.2012.07.042

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Cite this paper

@article{Jin2012ASF, title={A strategy for enrichment of the bioactive sphingoid base-1-phosphates produced by Hypericum perforatum L. in a balloon type airlift reactor.}, author={You-Xun Jin and Xi-Hua Cui and Kee-yoeup Paek and Y I Yim}, journal={Bioresource technology}, year={2012}, volume={123}, pages={284-9} }