A strategy for determining the orientations of refractory particles for reconstruction from cryo-electron micrographs with particular reference to round, smooth-surfaced, icosahedral viruses.

@article{Castn1999ASF,
  title={A strategy for determining the orientations of refractory particles for reconstruction from cryo-electron micrographs with particular reference to round, smooth-surfaced, icosahedral viruses.},
  author={Jos{\'e} R Cast{\'o}n and David M. Belnap and Alasdair C. Steven and Benes L Trus},
  journal={Journal of structural biology},
  year={1999},
  volume={125 2-3},
  pages={209-15}
}
Cryo-electron microscopy and three-dimensional image reconstruction are powerful tools for analyzing icosahedral virus capsids at resolutions that now extend below 1 nm. However, the validity of such density maps depends critically on correct identification of the viewing geometry of each particle in the data set. In some cases-for example, round capsids with low surface relief-it is difficult to identify orientations by conventional application of the two most widely used approaches-"common… CONTINUE READING