A stop-flow microperfusion technique for rapid determination of HCO3 – absorption/H+ secretion by isolated renal tubules

@article{MllerBerger1999ASM,
  title={A stop-flow microperfusion technique for rapid determination of HCO3 – absorption/H+ secretion by isolated renal tubules},
  author={Suzanne M{\"u}ller-Berger and Ita Samarzija and Motoei Kunimi and Hideomi Yamada and Eberhard Fr{\"o}mter and George Seki},
  journal={Pfl{\"u}gers Archiv},
  year={1999},
  volume={439},
  pages={208-215}
}
In the present experiments on microdissected tubules of rabbit kidney we present a refined stop-flow method for determining the rate of HCO3 – absorption (J HCO3) or H+ secretion (J H) that can be applied to isolated microperfused tubules. Using the pH-sensitive indicator dye BCECF (2',7'-bis [2-carboxyethyl]-5[6]-carboxyfluorescein) the luminal perfusate pH is continuously measured with a microspectrofluorometric set-up, and the pH change following a sudden stop of perfusion is analysed… CONTINUE READING
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