A stepwise approach for the reproducible optimization of PAMO expression in Escherichia coli for whole-cell biocatalysis

@inproceedings{Bloois2012ASA,
  title={A stepwise approach for the reproducible optimization of PAMO expression in Escherichia coli for whole-cell biocatalysis},
  author={Edwin van Bloois and Hanna M. Dudek and Wouter A. Duetz and Marco W Fraaije},
  booktitle={BMC biotechnology},
  year={2012}
}
BACKGROUND Baeyer-Villiger monooxygenases (BVMOs) represent a group of enzymes of considerable biotechnological relevance as illustrated by their growing use as biocatalyst in a variety of synthetic applications. However, due to their increased use the reproducible expression of BVMOs and other biotechnologically relevant enzymes has become a pressing… CONTINUE READING