A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor.

@article{Liu2015AST,
  title={A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor.},
  author={Bin Liu and Yi Pang and Rachida A. Bouhenni and Ernest Duah and Sailaja Paruchuri and Lucas J Mcdonald},
  journal={Chemical communications},
  year={2015},
  volume={51 55},
  pages={
          11060-3
        }
}
In this study, we report a series of novel flavone-based sensors that exhibit a superior fluorescence response when interacting with serum albumin in real serum samples and in acrylamide gels. The detection limit of probe 4 for serum albumin solution is 0.09 μg mL(-1), and the detectable volume for monkey serum reaches as low as 0.03 μL. 
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References

SHOWING 1-10 OF 41 REFERENCES
A highly sensitive “turn-on” fluorescent probe for bovine serum albumin protein detection and quantification based on AIE-active distyrylanthracene derivative
A sulfonated 9,10-distyrylanthracene derivative with aggregation-induced emission (AIE) property is designed and synthesized. It shows a highly sensitive and selective fluorescence enhancement… Expand
Site-selective binding and dual mode recognition of serum albumin by a squaraine dye.
TLDR
Employing displacement cum fluorimetry using site-specific binding ligands, such as dansylproline and dansylamide, indicated that SQ binds with protein selectively at site II involving hydrophobic, hydrogen bonding, and electrostatic interactions. Expand
The rational development of molecularly imprinted polymer-based sensors for protein detection.
TLDR
This critical review describes many methods used for imprinting recognition for protein targets in polymers and their incorporation with a number of transducer platforms with the aim of identifying the most promising approaches for the preparation of MIP-based protein sensors. Expand
Aggregation control of squaraines and their use as near-infrared fluorescent sensors for protein.
TLDR
While both H- and J-aggregates contribute to the observed fluorescence turn-on, the former appears to play a more important role and Transformation of the dye molecules from the aggregates to the monomeric species appears to be responsible for the large fluorescenceturn-on. Expand
Spectroscopic study of squaraines as protein-sensitive fluorescent dyes
Abstract A series of new symmetrical and asymmetrical squaraines were synthesised and efficiency of their use as fluorescent probes for the specific detection of proteins was studied.… Expand
Red‐Emissive Fluorescein Derivatives and Detection of Bovine Serum Albumin
Among them, red-emissionprobes are more desirable because red emission at wave-lengths longer than 600 nm is less prone to interferencefrom light scattering and the autofluorescence of… Expand
Interaction of 7-hydroxyflavone with human serum albumin: a spectroscopic study.
TLDR
Evidence for efficient Förster type resonance energy transfer (FRET, from tryptophan to 7HFA) is presented, and the near-UV circular dichroism studies show that the electronic transitions of 7HF are strongly perturbed on binding to the chiral host (HSA), leading to the appearance of ICD bands. Expand
The binding of novel two-color fluorescence probe FA to serum albumins of different species.
TLDR
The novel two-color ratiometric fluorescence probe FA belonging to a class of 3-hydroxychromone dyes was applied for characterization of binding sites in serum albumins obtained from seven species and was characterized by very low polarity, high electronic polarizability and different extent of intermolecular hydrogen bonding. Expand
Flavonoid-serum albumin complexation: determination of binding constants and binding sites by fluorescence spectroscopy.
TLDR
Overall, flavonoids display moderate affinities for albumins (binding constants in the range 1-15 x 10(4) M(-1), flavones and flavonols being most tightly bound), and it can be proposed that the binding of flavonol primarily takes place in subdomain IIA. Expand
Design and synthesis of intramolecular charge transfer-based fluorescent reagents for the highly-sensitive detection of proteins.
TLDR
The experimental results clearly showed that these fluorescent probes are good protein indicators for easy and highly sensitive detection. Expand
...
1
2
3
4
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