A step subsequent to preinitiation complex assembly at the ribosomal RNA gene promoter is rate limiting for human RNA polymerase I-dependent transcription.

@article{Panov2001ASS,
  title={A step subsequent to preinitiation complex assembly at the ribosomal RNA gene promoter is rate limiting for human RNA polymerase I-dependent transcription.},
  author={Kostya I. Panov and J Karsten Friedrich and Joost C. B. M. Zomerdijk},
  journal={Molecular and cellular biology},
  year={2001},
  volume={21 8},
  pages={2641-9}
}
The assembly, disassembly, and functional properties of transcription preinitiation complexes (PICs) of human RNA polymerase I (Pol I) play a crucial role in the regulation of rRNA gene expression. To study the factors and processes involved, an immobilized-promoter template assay has been developed that allows the isolation from nuclear extracts of functional PICs, which support accurate initiation of transcription. Immunoblotting of template-bound factors showed that these complexes contained… CONTINUE READING

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