A stem cell-specific silencer in the primer-binding site of a retrovirus.

@article{Petersen1991ASC,
  title={A stem cell-specific silencer in the primer-binding site of a retrovirus.},
  author={Robert O. Petersen and Gail Kempler and Eric Barklis},
  journal={Molecular and cellular biology},
  year={1991},
  volume={11 3},
  pages={1214-21}
}
Retrovirus expression in embryonal carcinoma (EC) cells is blocked at a postintegration stage of the viral life cycle, in part because of the inadequate function of the viral long terminal repeat promoter in this cell type. However, selection for retrovirus expression in EC cells has identified mutations in Moloney murine leukemia virus (M-MuLV) located in the tRNA primer-binding site (PBS) region which relieve the EC cell-specific repression. We have found that exchanging the M-MuLV proline… CONTINUE READING

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