A steady-state kinetic method for the verification of the rapid-equilibrium assumption in allosteric enzymes.

Abstract

A method for testing the validity of the rapid-equilibrium assumption as it might apply to allosteric enzymes using exclusively steady-state kinetic data is presented. The method is based upon a recognition that the ratio of apparent dissociation constants for the allosteric ligand, obtained under conditions of limiting and saturating substrate… (More)

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