A statistical approach to determine monoclonality after limiting cell plating of a hybridoma clone.

@article{Lietzke1985ASA,
  title={A statistical approach to determine monoclonality after limiting cell plating of a hybridoma clone.},
  author={Rolf Lietzke and Klaus Unsicker},
  journal={Journal of immunological methods},
  year={1985},
  volume={76 2},
  pages={223-8}
}
One of the standard methods to isolate a hybridoma clone producing a monoclonal antibody requires successive steps of limiting dilution. The probability of obtaining a monoclonal antibody increases with the number of limiting dilution steps. However, without meticulous visual screening monoclonality is hard to prove. Here we describe a statistical analysis, based on Poisson's approximation, which allows one to calculate the number of hybridoma cells at a given plating efficiency so that when… CONTINUE READING

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