A statistical analysis of random mutagenesis methods used for directed protein evolution.

  title={A statistical analysis of random mutagenesis methods used for directed protein evolution.},
  author={T. S. Wong and D. Roccatano and M. Zacharias and U. Schwaneberg},
  journal={Journal of molecular biology},
  volume={355 4},
  • T. S. Wong, D. Roccatano, +1 author U. Schwaneberg
  • Published 2006
  • Biology, Medicine
  • Journal of molecular biology
  • We have developed a statistical method named MAP (mutagenesis assistant program) to equip protein engineers with a tool to develop promising directed evolution strategies by comparing 19 mutagenesis methods. Instead of conventional transition/transversion bias indicators as benchmarks for comparison, we propose to use three indicators based on the subset of amino acid substitutions generated on the protein level: (1) protein structure indicator; (2) amino acid diversity indicator with a codon… CONTINUE READING
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