A statistical analysis of random mutagenesis methods used for directed protein evolution.
@article{Wong2006ASA, title={A statistical analysis of random mutagenesis methods used for directed protein evolution.}, author={T. S. Wong and D. Roccatano and M. Zacharias and U. Schwaneberg}, journal={Journal of molecular biology}, year={2006}, volume={355 4}, pages={ 858-71 } }
We have developed a statistical method named MAP (mutagenesis assistant program) to equip protein engineers with a tool to develop promising directed evolution strategies by comparing 19 mutagenesis methods. Instead of conventional transition/transversion bias indicators as benchmarks for comparison, we propose to use three indicators based on the subset of amino acid substitutions generated on the protein level: (1) protein structure indicator; (2) amino acid diversity indicator with a codon… CONTINUE READING
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