A statistical analysis of random mutagenesis methods used for directed protein evolution.

@article{Wong2006ASA,
  title={A statistical analysis of random mutagenesis methods used for directed protein evolution.},
  author={T. S. Wong and D. Roccatano and M. Zacharias and U. Schwaneberg},
  journal={Journal of molecular biology},
  year={2006},
  volume={355 4},
  pages={
          858-71
        }
}
  • T. S. Wong, D. Roccatano, +1 author U. Schwaneberg
  • Published 2006
  • Biology, Medicine
  • Journal of molecular biology
    • We have developed a statistical method named MAP (mutagenesis assistant program) to equip protein engineers with a tool to develop promising directed evolution strategies by comparing 19 mutagenesis methods. Instead of conventional transition/transversion bias indicators as benchmarks for comparison, we propose to use three indicators based on the subset of amino acid substitutions generated on the protein level: (1) protein structure indicator; (2) amino acid diversity indicator with a codon… CONTINUE READING
    View on PubMed
    classes.soe.ucsc.edu
    140 Citations
    Highly Influential Citations
    6
    Background Citations
    32
    Methods Citations
    20
    Results Citations
    2
    140 Citations
    Citation Type

    Citation Type

    The Mutagenesis Assistant Program.
    • 5
    MAP(2.0)3D: a sequence/structure based server for protein engineering.
    • 17
    Are transversion mutations better? A Mutagenesis Assistant Program analysis on P450 BM‐3 heme domain
    • 29
    Challenges of the genetic code for exploring sequence space in directed protein evolution
    • 29
    Enzyme Engineering by Directed Evolution
    • 2
    Directed Evolution of DNA Polymerases for Advancement of the SeSaM Mutagenesis Method and Biotransformations with P450 BM3 Monooxygenase
    Directed Evolution by Using Iterative Saturation Mutagenesis Based on Multiresidue Sites
    • 38
    A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons.
    • J. Liu, T. A. Cropp
    • Biology, Medicine
    • Protein engineering, design & selection : PEDS
    • 2012
    • 13
    • PDF
    Advancements of the Sequence Saturation Mutagenesis (SeSaM) Method to Efficiently Explore Protein Sequence Space
    • 1
    Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations
    • 32

    References

    SHOWING 1-10 OF 46 REFERENCES
    The diversity challenge in directed protein evolution.
    • 109
    A new approach to random mutagenesis in vitro
    • 45
    Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant.
    • 99
    A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.
    • 29
    Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization.
    • 149
    Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
    • 140
    • PDF
    Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR.
    • 103
    • PDF
    Sensitive Assay for Laboratory Evolution of Hydroxylases toward Aromatic and Heterocyclic Compounds
    • 49
    Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions.
    • 138
    • PDF
    The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    • 356